High_throughput_sequencing August 2010

high_throughput_sequencing@lists.fas.harvard.edu

4 participants
4 discussions

by Claire Reardon

Dear High Throughput Sequencing Users, Our next meeting will be Tuesday September 7th at 1pm in NWL room 425. Among other things, we'll discuss forthcoming updates to Illumina's software and reagents which will allow for longer, higher quality reads but will impact our ability to save image data. I hope to see you there. Claire Reardon Laboratory Manager FAS Center for Systems Biology Harvard University Northwest Lab room B239.2 52 Oxford St Cambridge, MA 02138 P: 617-384-7913 F: 617-496-0051 claire(a)cgr.harvard.edu

13 years, 9 months

any more long PE samples?

by Christian Daly

Is anyone working on 101 bp PE samples? I've got 5 coming in soon. -Christian

13 years, 9 months

KAPA QPCR kit pricing

by Claire Reardon

Dear All, At our meeting yesterday, I offered to look up pricing for the KAPA QPCR kits. The kit list price is $575 and includes 5ml of 2X master mix. With a large reaction volume of 25ul, you'd have enough material for 400 reactions making the cost/reaction $1.44. At a smaller reaction volume of 10ul, the cost would drop to $0.58 per reaction. Plan on performing a few standards in duplicate for each assay, giving a cost of about $10 to $20 to quantify a library. Add to this the cost of training on the QPCR instrument ($124) and the cost of instrument use for a 3 hour run ($12). Remember that you can run a QPCR quantification assay with any QPCR reagents - you do not need to purchase the KAPA kit. The convenience of a kit is that the primers and standards are included in one package along with the master mix. I hope this helps. Claire Reardon Laboratory Manager FAS Center for Systems Biology Harvard University Northwest Lab room B239.2 52 Oxford St Cambridge, MA 02138 P: 617-384-7913 F: 617-496-0051 claire(a)cgr.harvard.edu

13 years, 10 months

NGS analysis course link

by Claire Reardon

Dear All, Please see the email below from Brant Peterson about a course offered on NGS data analysis. Best, Claire -----Original Message----- From: Brant Peterson [mailto:brantp@gmail.com] Sent: Monday, August 02, 2010 8:03 PM To: Claire Reardon Subject: link Claire, This is the link to a next-gen sequencing course taught this summer that collects a lot of straightforward examples of basic analysis of NGS data that's explicitly targeted to new-to-programming biologists: http://ged.msu.edu/angus/ If it looks to you like it would be useful, feel free to send it around! -Brant

13 years, 10 months

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High_throughput_sequencing August 2010