***HQOC/ITAMP Joint Quantum Sciences Seminar***
**April 17, 2013 - 4:00 PM - Jefferson 250**
*Prof. Harald Hess, HHMI - Janelia Farm Research Campus*
"3D Imaging at the Limits with Photo-Activated Labels and Electrons"
Fluorescence microscopy, is limited in its ability to resolve densely labeled features
smaller than the optical diffraction limit, however special photoactivated fluorescent
proteins or dyes can be harnessed in a technique called Photo-Activated Localization
Microscopy, PALM. A series of personal challenges in fiber optics, fly height testing, and
high throughput disk drive screening provided a diverse foundation in interferometry that
inspired a new 3D imaging concept. This multiphase interferometry one can measure the
vertical position of fluorescent molecules to nanometer precision with the highest photon
efficiency and can be combined with PALM to give full 3 dimensional molecular coordinates
of genetically tagged proteins with ~ 10-20 nm resolution. In a complimentary 3D project,
electron microscopes, EM, can be customized for high throughput imaging for neural tissue
or cells. Correlating PALM and electron microscopy gives EM images, which can be
colorized with specific protein locations.
Student Presentation by Yat Shan Au, Graduate Student, Doyle Lab
Student Presentation will begin at 4:00 PM
Guest Presentation will begin at 4:30 PM
Refreshments will be provided
Joan Hamilton
Faculty Assistant to Profs. Greiner and Lukin
HQOC Laboratory Administrator
HUCTW Local Union Representative
Harvard University
Department of Physics
17 Oxford Street
Cambridge, MA 02138
P: (617) 496-2544
F: (617) 496-2545
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***HQOC/ITAMP Joint Quantum Sciences Seminar***
**April 17, 2013 - 4:00 PM - Jefferson 250**
*Prof. Harald Hess, HHMI - Janelia Farm Research Campus*
"3D Imaging at the Limits with Photo-Activated Labels and Electrons"
Fluorescence microscopy, is limited in its ability to resolve densely labeled features
smaller than the optical diffraction limit, however special photoactivated fluorescent
proteins or dyes can be harnessed in a technique called Photo-Activated Localization
Microscopy, PALM. A series of personal challenges in fiber optics, fly height testing, and
high throughput disk drive screening provided a diverse foundation in interferometry that
inspired a new 3D imaging concept. This multiphase interferometry one can measure the
vertical position of fluorescent molecules to nanometer precision with the highest photon
efficiency and can be combined with PALM to give full 3 dimensional molecular coordinates
of genetically tagged proteins with ~ 10-20 nm resolution. In a complimentary 3D project,
electron microscopes, EM, can be customized for high throughput imaging for neural tissue
or cells. Correlating PALM and electron microscopy gives EM images, which can be
colorized with specific protein locations.
Student Presentation by Yat Shan Au, Graduate Student, Doyle Lab
"Cold Collision Studies with ThO"
Student Presentation will begin at 4:00 PM
Guest Presentation will begin at 4:30 PM
Refreshments will be provided
Joan Hamilton
Faculty Assistant to Profs. Greiner and Lukin
HQOC Laboratory Administrator
HUCTW Local Union Representative
Harvard University
Department of Physics
17 Oxford Street
Cambridge, MA 02138
P: (617) 496-2544
F: (617) 496-2545